Discovery Creek Children's Museum works with several key partners to carry out its mission of encouraging all children to experience, appreciate, and become stewards of the natural environment. They include:
Academy for Educational Development
American Association of Museums
Association of Children's Museums
Chesapeake Bay Foundation
Children's National Medical Center
Co-op America
DC Children and Youth Investment Trust Corporation
DC Department of Parks and Recreation
DC Energy Office - Green DC Week
DC Housing Authority
District of Columbia Environmental Education Consortium
District of Columbia Public Elementary Schools
Girl Scout Council of the Nation’s Capital
Glen Echo Park Partnership for Arts & Culture
Montgomery County
National Capital Area Council, Boy Scouts of America
National Capital Parks – East
National Maritime Heritage Foundation
National Park Service, Department of the Interior
Northern Virginia Regional Park Authority
Project Learning Tree
Smithsonian Environmental Research Center
Zipcar